ASTM E3011-22 - Standard Practice for In vitro production of <emph type="ital">Clostridioides difficile</emph > Spores
Standard Practice for In vitro production of <emph type="ital">Clostridioides difficile</emph > Spores
| Standard number: | E3011-22 |
| Released: | 01.10.2022 |
| Status: | Active |
| Pages: | 3 |
| Section: | 11.08 |
| Keywords: | C. difficile; Clostridioides; liver broth; propagation; spores; spore crop; sporulation; |
1.1 This practice is designed to propagate spores of Clostridioides difficile using liver broth.
1.2 It is the responsibility of the user of this practice to determine whether Good Laboratory Practices are required and follow when appropriate.
1.3 This practice should only be performed by those trained in microbiological techniques.
1.4 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.