ASTM E3285-22 - Standard Test Method for Evaluation of Contact-Mediated Microbial Transference

1.1 This test method is designed to evaluate the contact-mediated microbial transference on two-dimensional material surfaces. Material surfaces intended to reduce microorganism adherence or contamination may be evaluated using this test method. Additionally, this test method can be utilized on a wide variety of material surfaces for other downstream processes.

1.2 This test method is designed to quantify the fomite transfer of bacteria on material surfaces through touch-transfer, be inexpensive, require limited training in practice, and be adaptable to many surfaces or microorganisms. Non-porous surfaces are contaminated with inoculated filter paper as a carrier and surface contamination is quantified by sampling with Replicate Organism Detection and Counting (RODAC) plates.

1.3 Basic microbiology training is required to perform this test method.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.