BS EN ISO 15216-1:2017+A1:2021

Microbiology of the Food Chain: Horizontal Method for Determination of Hepatitis A Virus and Norovirus Using Real-Time RT-PCR Method for Quantification

In the ever-evolving world of food safety, ensuring the microbiological integrity of the food chain is paramount. The BS EN ISO 15216-1:2017+A1:2021 standard is a crucial tool for professionals in the food industry, laboratories, and regulatory bodies. This comprehensive document provides a horizontal method for the determination of hepatitis A virus and norovirus using the advanced real-time RT-PCR method for quantification.

Key Features of the Standard

• Standard Number: BS EN ISO 15216-1:2017+A1:2021
• Pages: 54
• Released: April 30, 2021
• ISBN: 978 0 539 13468 1
• Status: Standard

Why This Standard is Essential

Foodborne viruses, particularly hepatitis A virus and norovirus, are significant causes of foodborne illnesses worldwide. These viruses can lead to severe health issues, and their presence in the food chain poses a substantial risk to public health. The BS EN ISO 15216-1:2017+A1:2021 standard provides a reliable and validated method for detecting and quantifying these viruses, ensuring that food products are safe for consumption.

The real-time RT-PCR method outlined in this standard is a state-of-the-art technique that offers high sensitivity and specificity. It allows for the rapid detection of viral RNA, making it an invaluable tool for food safety testing. By implementing this method, laboratories can ensure accurate and timely results, which are critical for preventing outbreaks and protecting consumers.

Comprehensive Coverage

This 54-page document is meticulously crafted to cover all aspects of the testing process. It includes detailed instructions on sample preparation, RNA extraction, and the real-time RT-PCR procedure. The standard also provides guidance on interpreting results, ensuring that users can confidently assess the presence of hepatitis A virus and norovirus in food samples.

Moreover, the standard is designed to be applicable across various types of food matrices, making it a versatile tool for different sectors of the food industry. Whether you are testing shellfish, fresh produce, or ready-to-eat meals, this standard provides the necessary framework to ensure food safety.

Benefits of Using BS EN ISO 15216-1:2017+A1:2021

• Enhanced Food Safety: By accurately detecting and quantifying viruses, this standard helps prevent foodborne illnesses and ensures consumer safety.
• Regulatory Compliance: Adhering to this internationally recognized standard demonstrates compliance with food safety regulations and enhances your organization's credibility.
• Improved Testing Efficiency: The real-time RT-PCR method is a rapid and efficient testing technique, allowing for timely decision-making and response to potential contamination.
• Versatility: Applicable to a wide range of food products, this standard is suitable for various sectors within the food industry.

Who Should Use This Standard?

The BS EN ISO 15216-1:2017+A1:2021 standard is an essential resource for:

• Food Safety Laboratories: Laboratories conducting microbiological testing of food products will benefit from the detailed procedures and guidelines provided in this standard.
• Food Manufacturers: Companies involved in the production and processing of food can use this standard to ensure their products meet safety standards and are free from viral contamination.
• Regulatory Bodies: Government agencies and organizations responsible for food safety regulations can use this standard to establish testing protocols and ensure compliance across the industry.
• Quality Assurance Professionals: QA teams within food companies can implement this standard to enhance their food safety management systems and protect their brand reputation.

Conclusion

The BS EN ISO 15216-1:2017+A1:2021 standard is a vital tool in the fight against foodborne viruses. By providing a reliable and validated method for the detection and quantification of hepatitis A virus and norovirus, it plays a crucial role in safeguarding public health. Whether you are a laboratory professional, a food manufacturer, or a regulatory body, this standard offers the guidance and assurance needed to maintain the highest levels of food safety.

Invest in the BS EN ISO 15216-1:2017+A1:2021 standard today and take a proactive step towards ensuring the safety and quality of the food chain.

BS EN ISO 15216-1:2017+A1:2021

This standard BS EN ISO 15216-1:2017+A1:2021 Microbiology of the food chain. Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR is classified in these ICS categories:

• 07.100.30 Food microbiology

This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.