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Homepage>ISO Standards>ISO 8128-2:1993-Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content-Part 2: Method using thin-layer chromatography
download between 0-24 hoursReleased: 1993
ISO 8128-2:1993-Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content-Part 2: Method using thin-layer chromatography

ISO 8128-2:1993

ISO 8128-2:1993-Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content-Part 2: Method using thin-layer chromatography

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Standard´s number:ISO 8128-2:1993
Pages:5
Edition:1
Released:1993
DESCRIPTION

ISO 8128-2:1993


The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.
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