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immediate downloadReleased: 2016-08-31
PD ISO/TS 19006:2016
Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
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| Standard number: | PD ISO/TS 19006:2016 |
| Pages: | 32 |
| Released: | 2016-08-31 |
| ISBN: | 978 0 580 83224 6 |
| Status: | Standard |
DESCRIPTION
PD ISO/TS 19006:2016
This standard PD ISO/TS 19006:2016 Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line is classified in these ICS categories:
- 07.120 Nanotechnologies
This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.