PD ISO/TS 20224-1:2020: Molecular Biomarker Analysis

Detection of Animal-Derived Materials in Foodstuffs and Feedstuffs by Real-Time PCR Bovine DNA Detection Method

In the ever-evolving world of food safety and quality assurance, the PD ISO/TS 20224-1:2020 standard stands as a beacon of precision and reliability. This comprehensive standard is designed to guide laboratories and food safety professionals in the detection of animal-derived materials, specifically bovine DNA, in foodstuffs and feedstuffs using the advanced technique of real-time PCR (Polymerase Chain Reaction).

Key Features and Benefits

• Standard Number: PD ISO/TS 20224-1:2020
• Pages: 26
• Release Date: August 13, 2020
• ISBN: 978 0 539 04892 6
• Status: Standard

This standard is an essential tool for ensuring the integrity and authenticity of food products. It provides a robust framework for the detection of bovine DNA, which is crucial for verifying the presence of animal-derived materials in various food and feed products. This is particularly important for industries where the accurate labeling of products is critical, such as in the production of vegetarian or vegan foods, or in regions where certain animal products are restricted or prohibited.

Why Choose PD ISO/TS 20224-1:2020?

The PD ISO/TS 20224-1:2020 standard is meticulously crafted to meet the highest international standards of food safety and quality. By implementing this standard, laboratories can ensure that their testing methods are not only accurate but also consistent with global best practices. This is vital for maintaining consumer trust and meeting regulatory requirements.

Advanced Real-Time PCR Methodology

The real-time PCR method outlined in this standard is a cutting-edge technique that allows for the rapid and precise detection of bovine DNA. This method is highly sensitive and specific, making it an invaluable tool for food safety testing. The use of real-time PCR technology ensures that results are obtained quickly, allowing for timely decision-making in the management of food safety risks.

Comprehensive Guidance

With 26 pages of detailed instructions and guidelines, this standard provides comprehensive coverage of the entire testing process. From sample preparation to data analysis, every step is clearly outlined to ensure that laboratories can implement the method with confidence and accuracy.

Global Applicability

As a recognized international standard, PD ISO/TS 20224-1:2020 is applicable across various regions and industries. This makes it an ideal choice for multinational companies and laboratories that need to comply with diverse regulatory requirements. By adhering to this standard, organizations can demonstrate their commitment to food safety and quality on a global scale.

Applications of the Standard

The applications of the PD ISO/TS 20224-1:2020 standard are vast and varied. It is particularly useful in the following areas:

• Food Industry: Ensuring the accurate labeling of products and verifying the absence of bovine materials in vegetarian or vegan products.
• Feed Industry: Detecting the presence of bovine DNA in animal feed to prevent cross-contamination and ensure compliance with feed safety regulations.
• Regulatory Bodies: Providing a reliable method for the enforcement of food safety regulations and standards.
• Research Institutions: Supporting scientific research in the field of food safety and molecular biology.

Conclusion

The PD ISO/TS 20224-1:2020 standard is an indispensable resource for any organization involved in the production, testing, or regulation of food and feed products. Its rigorous methodology and comprehensive guidelines ensure that laboratories can achieve the highest levels of accuracy and reliability in their testing processes. By adopting this standard, organizations can enhance their food safety protocols, protect consumer health, and maintain the integrity of their products.

Invest in the PD ISO/TS 20224-1:2020 standard today and take a significant step towards ensuring the safety and quality of your food and feed products. With its advanced real-time PCR methodology and global applicability, this standard is the key to unlocking a new level of precision and trust in your testing processes.

PD ISO/TS 20224-1:2020

This standard PD ISO/TS 20224-1:2020 Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR is classified in these ICS categories:

• 67.050 General methods of tests and analysis for food products

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of bovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of bovine material derived from cattle including taurine (Bos taurus) and zebu (Bos indicus). The assay also detects the species bison (Bison bison) and yak (Bos mutus).

The target sequence is a partial fragment of the bovine nuclear beta actin gene in chromosome 25 (i.e. GenBank accession number NC_037352.1)^{[ 1][ 2][ 3]}, which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD_95 %).