PD ISO/TS 20224-2:2020 - Molecular Biomarker Analysis

In the ever-evolving world of food safety and quality assurance, the ability to accurately detect animal-derived materials in foodstuffs and feedstuffs is paramount. Introducing the PD ISO/TS 20224-2:2020, a cutting-edge standard that provides a comprehensive method for the detection of ovine DNA using real-time PCR technology. This standard is an essential tool for laboratories, food manufacturers, and regulatory bodies committed to ensuring the integrity and safety of food products.

Overview of the Standard

The PD ISO/TS 20224-2:2020 is a technical specification that outlines a precise methodology for the molecular biomarker analysis of animal-derived materials. Specifically, it focuses on the detection of ovine (sheep) DNA in various food and feed products. This standard is part of a series that addresses the need for reliable and reproducible testing methods in the food industry.

Key Features:

• Standard Number: PD ISO/TS 20224-2:2020
• Pages: 26
• Released: August 13, 2020
• ISBN: 978 0 539 04893 3
• Status: Standard

Why Choose PD ISO/TS 20224-2:2020?

This standard is indispensable for several reasons:

1. Precision and Reliability

The PD ISO/TS 20224-2:2020 employs real-time PCR, a highly sensitive and specific technique that allows for the accurate detection of ovine DNA. This ensures that even trace amounts of animal-derived materials can be identified, providing confidence in the results.

2. Compliance and Regulation

With increasing regulatory demands for transparency and traceability in the food supply chain, this standard helps organizations comply with national and international regulations. It supports the verification of labeling claims and the detection of potential contamination or adulteration in food products.

3. Versatility and Application

The methodology outlined in this standard is applicable to a wide range of foodstuffs and feedstuffs. Whether you are testing raw materials, processed foods, or animal feeds, the PD ISO/TS 20224-2:2020 provides a robust framework for analysis.

4. Industry Relevance

As consumer demand for transparency and ethical sourcing grows, the ability to verify the presence of animal-derived materials is increasingly important. This standard supports the food industry's commitment to quality assurance and consumer trust.

Technical Insights

The PD ISO/TS 20224-2:2020 standard is meticulously designed to guide laboratories through the process of detecting ovine DNA. It includes detailed instructions on sample preparation, DNA extraction, and the real-time PCR process. The standard also provides criteria for the interpretation of results, ensuring consistency and accuracy across different testing environments.

Real-Time PCR Technology

Real-time PCR is a powerful tool in molecular biology, allowing for the amplification and quantification of DNA sequences. This technology is particularly suited for the detection of specific DNA markers, such as those derived from ovine sources. The PD ISO/TS 20224-2:2020 leverages this technology to deliver precise and reliable results.

Who Should Use This Standard?

The PD ISO/TS 20224-2:2020 is ideal for:

• Food Testing Laboratories: Laboratories that specialize in food safety testing will find this standard invaluable for its detailed methodology and reliable results.
• Food Manufacturers: Companies that produce or process food products can use this standard to verify the integrity of their supply chain and ensure compliance with labeling requirements.
• Regulatory Bodies: Government agencies and regulatory organizations can adopt this standard to enforce food safety regulations and protect consumer interests.
• Quality Assurance Professionals: QA teams within the food industry can utilize this standard to enhance their testing protocols and maintain high standards of product quality.

Conclusion

In a world where food safety and authenticity are of utmost importance, the PD ISO/TS 20224-2:2020 stands out as a vital resource for detecting animal-derived materials in foodstuffs and feedstuffs. Its precise methodology, based on real-time PCR technology, ensures accurate and reliable results, making it an essential tool for laboratories, manufacturers, and regulatory bodies alike. By adopting this standard, organizations can enhance their testing capabilities, comply with regulatory requirements, and ultimately, protect consumer trust and safety.

PD ISO/TS 20224-2:2020

This standard PD ISO/TS 20224-2:2020 Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR is classified in these ICS categories:

• 67.050 General methods of tests and analysis for food products

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of ovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of ovine material derived from sheep (Ovis aries).

The target sequence is a partial fragment of the ovine nuclear prolactin receptor short form mRNA gene (PRLR) (i.e. GenBank accession number AF041979.1)^{[ 1]}, which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD_95 %).