PD ISO/TS 20224-7:2020: Molecular Biomarker Analysis

Detection of Animal-Derived Materials in Foodstuffs and Feedstuffs by Real-Time PCR Donkey DNA Detection Method

In the ever-evolving world of food safety and quality assurance, the need for precise and reliable methods to detect animal-derived materials in foodstuffs and feedstuffs is paramount. The PD ISO/TS 20224-7:2020 standard offers a cutting-edge solution for the detection of donkey DNA using real-time PCR technology. This standard is an essential tool for laboratories, food manufacturers, and regulatory bodies committed to ensuring the integrity and safety of food products.

Key Features of the PD ISO/TS 20224-7:2020 Standard

• Standard Number: PD ISO/TS 20224-7:2020
• Pages: 24
• Released: August 13, 2020
• ISBN: 978 0 539 04898 8
• Status: Standard

This standard provides a comprehensive methodology for the detection of donkey DNA in various food and feed products. It is designed to be used by professionals in the field of molecular biomarker analysis, offering a reliable and validated approach to ensure the authenticity and safety of products.

Why Choose Real-Time PCR for Donkey DNA Detection?

Real-time PCR is a powerful and sensitive technique that allows for the rapid and accurate detection of specific DNA sequences. This method is particularly advantageous for detecting low levels of animal-derived materials in complex food matrices. The PD ISO/TS 20224-7:2020 standard leverages the strengths of real-time PCR to provide a robust framework for donkey DNA detection, ensuring high specificity and sensitivity.

Applications of the PD ISO/TS 20224-7:2020 Standard

The applications of this standard are vast and varied, making it an invaluable resource for a range of industries:

• Food Industry: Ensure the authenticity of meat products and prevent food fraud by accurately identifying the presence of donkey DNA.
• Feed Industry: Verify the composition of animal feed to maintain quality and compliance with regulatory standards.
• Regulatory Bodies: Implement standardized testing protocols to uphold food safety regulations and protect consumer interests.
• Research Laboratories: Utilize the standard as a reference for developing new assays and conducting research in molecular biomarker analysis.

Benefits of Implementing the PD ISO/TS 20224-7:2020 Standard

Adopting this standard offers numerous benefits, including:

• Enhanced Food Safety: Protect consumers by ensuring that food products are free from undeclared animal-derived materials.
• Regulatory Compliance: Meet national and international food safety standards with a validated and recognized testing method.
• Consumer Confidence: Build trust with consumers by demonstrating a commitment to transparency and product integrity.
• Operational Efficiency: Streamline testing processes with a standardized method that reduces the risk of errors and inconsistencies.

Conclusion

The PD ISO/TS 20224-7:2020 standard is a vital resource for any organization involved in the production, regulation, or analysis of food and feed products. By providing a reliable method for detecting donkey DNA, this standard helps ensure the authenticity and safety of products, protecting both consumers and businesses alike. Embrace the future of food safety with this essential tool for molecular biomarker analysis.

PD ISO/TS 20224-7:2020

This standard PD ISO/TS 20224-7:2020 Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR is classified in these ICS categories:

• 67.050 General methods of tests and analysis for food products

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of donkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of donkey material derived from donkey (Equus asinus), mule (Equus caballus ? × Equus asinus ?) and hinny (Equus caballus ? × Equus asinus ?). The assay also detects the species zebra (Equus burchellii).

The target sequence is a partial fragment of the Equus asinus isolate Maral har breed Guanzhong donkey unplaced genomic scaffold, ASM130575v1 scaffold786, whole genome shotgun sequence (i.e. GenBank accession number NW_014638576.1)^{[ 1]}, which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD_95 %).