PD ISO/TS 21569-3:2020 - Horizontal Methods for Molecular Biomarker Analysis

In the rapidly evolving field of biotechnology, the ability to accurately detect and analyze genetically modified organisms (GMOs) is crucial. The PD ISO/TS 21569-3:2020 standard provides a comprehensive framework for the detection of GMOs and their derived products using a construct-specific real-time PCR method. This standard is an essential tool for laboratories, researchers, and regulatory bodies involved in GMO screening and analysis.

Overview of the Standard

The PD ISO/TS 21569-3:2020 standard outlines horizontal methods for molecular biomarker analysis, specifically focusing on the detection of the P35S-pat-sequence. This sequence is a common marker used in the screening of genetically modified organisms. The standard provides detailed methodologies for conducting real-time PCR, a powerful technique that allows for the precise and efficient detection of specific genetic sequences.

Key Features

• Standard Number: PD ISO/TS 21569-3:2020
• Pages: 22
• Released: 2020-07-08
• ISBN: 978 0 539 12444 6
• Status: Standard

Why Choose PD ISO/TS 21569-3:2020?

This standard is designed to meet the needs of professionals in the field of molecular biology and biotechnology. It provides a reliable and validated method for the detection of GMOs, ensuring that laboratories can maintain high standards of accuracy and consistency in their analyses. By adhering to this standard, organizations can enhance their credibility and ensure compliance with international regulations.

Benefits of Using This Standard

• Accuracy: The real-time PCR method described in this standard offers high sensitivity and specificity, allowing for the accurate detection of GMOs.
• Efficiency: The methodologies outlined are designed to streamline the analysis process, reducing time and resource expenditure.
• Compliance: Adhering to this standard helps ensure compliance with international guidelines and regulations regarding GMO detection.
• Reproducibility: The standardized approach ensures that results are consistent and reproducible across different laboratories and studies.

Applications

The PD ISO/TS 21569-3:2020 standard is applicable in various sectors, including:

• Food and Agriculture: Ensuring that food products are accurately labeled and free from unauthorized GMOs.
• Environmental Monitoring: Detecting and monitoring the presence of GMOs in the environment to assess ecological impacts.
• Research and Development: Supporting scientific studies and innovations in the field of genetic engineering.
• Regulatory Compliance: Assisting regulatory bodies in enforcing GMO-related laws and standards.

Conclusion

The PD ISO/TS 21569-3:2020 standard is an indispensable resource for anyone involved in the detection and analysis of genetically modified organisms. Its comprehensive guidelines and methodologies ensure that users can achieve accurate, efficient, and compliant results. By implementing this standard, laboratories and organizations can enhance their operational capabilities and contribute to the responsible management of GMOs in various industries.

For those seeking to maintain the highest standards in molecular biomarker analysis, the PD ISO/TS 21569-3:2020 is the definitive choice. Embrace this standard to ensure precision, reliability, and compliance in your GMO detection efforts.

PD ISO/TS 21569-3:2020

This standard PD ISO/TS 21569-3:2020 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products is classified in these ICS categories:

• 67.050 General methods of tests and analysis for food products

This document describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.

This document is applicable to the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate quantity and quality of amplifiable DNA from the relevant matrix.